Submitted: 09 Feb 2017
Accepted: 12 May 2017
First published online: 23 May 2017
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Avicenna J Med Biochem. 2017;5(1):1-8.
doi: 10.15171/ajmb.2017.01
  Abstract View: 308
  PDF Download: 319
  Full Text View: 226

Research Article

Different Expression and Localization of Phosphoinositide Specific Phospholipases C in Human Osteoblasts, Osteosarcoma Cell Lines, Ewing Sarcoma and Synovial Sarcoma

Vincenza Rita Lo Vasco 1 * , Martina Leopizzi 2, Anna Scotto d’Abusco 3, Carlo Della Rocca 2

1 Department of Sensory Organs, Sapienza University of Rome, Rome, Italy
2 Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino –Sapienza University, Latina, Rome, Italy
3 Department of Biochemical Sciences A. Rossi Fanelli, Sapienza University of Rome, Rome, Italy
*Corresponding Author: Vincenza Rita Lo Vasco, MD, PhD; Email: ritalovasco@hotmail.it

Abstract

Background: Bone hardness and strength depends on mineralization, which involves a complex process in which calcium phosphate, produced by bone-forming cells, was shed around the fibrous matrix. This process is strictly regulated, and a number of signal transduction systems were interested in calcium metabolism, such as the phosphoinositide (PI) pathway and related phospholipase C (PLC) enzymes.

Objectives: Our aim was to search for common patterns of expression in osteoblasts, as well as in ES and SS.

Methods: We analysed the PLC enzymes in human osteoblasts and osteosarcoma cell lines MG-63 and SaOS-2. We compared the obtained results to the expression of PLCs in samples of patients affected with Ewing sarcoma (ES) and synovial sarcoma (SS).

Results: In osteoblasts, MG-63 cells and SaOS-2 significant differences were identified in the expression of PLC δ4 and PLC η subfamily isoforms. Differences were also identified regarding the expression of PLCs in ES and SS. Most ES and SS did not express PLCB1, which was expressed in most osteoblasts, MG-63 and SaOS-2 cells. Conversely, PLCB2, unexpressed in the cell lines, was expressed in some ES and SS. However, PLCH1 was expressed in SaOS-2 and inconstantly expressed in osteoblasts, while it was expressed in ES and unexpressed in SS. The most relevant difference observed in ES compared to SS regarded PLC ε and PLC η isoforms.

Conclusion: MG-63 and SaOS-2 osteosarcoma cell lines might represent an inappropriate experimental model for studies about the analysis of signal transduction in osteoblasts

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