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Submitted: 08 Jul 2015
Revision: 27 Sep 2015
Accepted: 11 Oct 2015
ePublished: 23 Jan 2016
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Avicenna J Med Biochem. 2016;4(1): 3-30943.
doi: 10.17795/ajmb-30943
  Abstract View: 1747
  PDF Download: 1172
  Full Text View: 1287

Research Article

Assessment of Behavior of Rice Root Peroxidase in the Presence of Silver Nanoparticles

Somaye Mohammadzade 1, Arezoo Beig Parikhani 1, Hossein Askari 1*

1 Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University (G.C), Tehran, IR Iran
*Corresponding Author: *Corresponding author: Hossein Askari, Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University (G.C), Tehran, IR Iran, , Email: askarihossein@yahoo.com

Abstract

Background: Silver Nanoparticles (AgNPs) can change proteins function and structure. The increased production and high surface reactivity of silver nanoparticles, has interested researchers to study the interactions of these particles with biomolecules.

Objectives: The present study aimed to show the effects of AgNPs on rice plant root peroxidase enzyme and the interaction quality between silver nanoparticles and the enzyme.
Materials and Methods: Extracted peroxidase enzyme of rice plant root was treated by AgNPs at  concentrations of 0, 20, 40, 80, 100mg/L for 2, 7 and 24 hours. The experiment was done with 15 treatments for measuring the peroxidase enzyme activity using the spectrophotometry method at a wavelength of 470.

Results: Lowconcentrations of AgNPsandshort incubation times can have themaximumpositive impactonthe peroxidase activity, and in the present study the highest activity was seen at a concentration of 40 mg/L and two hours of incubation time.

Conclusions: This study suggests that changes of enzymeactivity can occur as a result of the effect of silver nanoparticlesonenzyme conformation, increase of reactive environment pH, and amount of substrate and enzyme stability.

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Abstract View: 1748

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