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Submitted: 02 May 2020
Revision: 23 May 2020
Accepted: 20 Jun 2020
ePublished: 29 Jun 2020
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Avicenna J Med Biochem. 2020;8(1): 27-34.
doi: 10.34172/ajmb.2020.04
  Abstract View: 1188
  PDF Download: 706

Research Article

Effect of Eight Weeks of Resistance Training and Consumption of Tribulus Terrestris on Androgenic Receptor-1, Fas Ligand Gene Expression, and Lipid Profiles in Rats Exposed to Stanozolol

Mohammad Derakhshandeh 1 ORCID logo, Farzaneh Taghian 1* ORCID logo, Khosro Jalali Dehkordi 1 ORCID logo, Seyed Ali Hosseini 2 ORCID logo

1 Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
2 Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
*Corresponding Author: *Corresponding author: Farzaneh Taghian, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. Tel: 00989133080241, Email: , Email: f_taghian@yahoo.com

Abstract

Background: Protective effect of medicinal plants on the heart has been reported, but the effect of resistance training (RT) and Tribulus terrestris (TT) on the heart exposed to anabolic-androgenic steroids (AAS) abuse is still unknown.

Objectives: The present study aimed to investigate the effect of RT and TT on androgen receptor-1 (ar-1), Fas ligand (fasl) gene expression and lipid profiles in rats exposed to stanozolol (S).

Methods: Thirty-five male rats were selected and divided into 7 groups as follows: (1) sham (normal saline/Sh), (2) stanozolol (S), (3) S+100 mg/kg TT (S+TT100), (4) S+ 50 mg/kg TT (S+TT50), (5) S+RT+TT, (6) S+RT+TT100, and (7) S+RT+TT50. Over a course of eight-week period, groups 3, 4, 6, and 7 received 50 and 100 mg/kg/d doses of TT peritoneally and groups 5-7 performed three sessions of increasing RT per week.

Results: RT decreased plasma cholesterol and low-density lipoprotein cholesterol (LDL-C) levels, as well as ar-1 and fasl gene expression in S-exposed rats (P<0.05). TT50, TT100, SRTT100, and SRTT50 reduced ar-1 and fasl gene expressions (P<0.05). TT50 reduced triglyceride (TG), cholesterol and increased high-density lipoprotein-cholesterol (HDL-C) (P≤0.01), and TT100 decreased LDL-C levels (P<0.05). Additionally, SRTT100 reduced TG, cholesterol, and LDL-C levels and increased HDL-C level (P<0.05), and SRTT50 decreased cholesterol level and increased HDL-C level in S-exposed rats (P<0.05).

Conclusion: RT and consumption of TT appear to have protective effects on the improvement of apoptosisdependent androgen receptor-1 and lipid profile in S-exposed rats.

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