Logo-ajmb
Submitted: 27 Jul 2021
Revision: 03 Sep 2021
Accepted: 15 Dec 2021
ePublished: 29 Dec 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Avicenna J Med Biochem. 2021;9: 59-64.
doi: 10.34172/ajmb.2021.11
  Abstract View: 131
  PDF Download: 121

Research Article

Anti-nociceptive Activity of Quebracho tannin Extract on Pain Induced by Formalin and Writhing Tests in Mice

Fatemeh Zare 1, Shahin Hassanpour 2* ORCID logo, Ahmad Asghari 3, Alireza Jahandideh 3

1 Graduate Student, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
*Corresponding Author: Corresponding author: Shahin Hassanpour, Tel: +98-21-44868560 Fax: +98-21-44868560, Postal Box: 1477893855, Mobile: +98-9129622545, Email: s.hassanpour@srbiau. ac.ir, , Email: hassanpour.shahin@gmail.com

Abstract

Background: Based on positive role of the tannins for pain relief, there is no report for possible antinociceptive activity of the Quebracho tannin. Objectives: This study aimed to determine the anti-nociceptive activity of the Quebracho tannin extract (QTE) on pain in mice.

Materials and Methods: For this purpose, 340 mice were used for formalin and writhing tests each including 4 experiments with 4 sub-groups. In experiment 1, mice were injected with saline, QTE (100 mg/kg), QTE (200 mg/kg), QTE (400 mg/kg), and morphine (5 mg/kg). In the second experiment, injections included saline, QTE (400 mg/kg), naloxone (2 mg/kg), and QTE + naloxone. Experiments 3 and 4 were similar to experiment 2, except that mice injected were with NG-nitro arginine methyl ester (L-NAME, 10 mg/kg) and cyproheptadine (4 mg/kg) instead of naloxone. Then, formalin (1%) was injected, and time spent for licking the injected paw was recorded until 30 minutes following injection in the first and second phases. Finally, injections in 4 experiment groups were the same, and animals were intraperitoneally injected with acetic acid, and contractions were recorded in the writhing test category.

Results: According to the results, QTE (100, 200, and 400 mg/kg) decreased pain in the injected paw (P=0.001) and inhibited the pain response by 59.37% (P=0.001). Moreover, the injection of naloxone + QTE significantly decreased pain in the injected paw (P=0.021). Eventually, the injection of the L-NAME + QTE significantly reduced the anti-nociception effect of the QTE on the formalin test (P=0.031) and writhing contractions (55.75%, P=0.033).

Conclusion: These findings suggested anti-nociceptive properties of the QTE mediated by opioidergic and nitrergic systems.

Keywords: Quebracho tannin, Pain, Formalin test, Mice,
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 132

Your browser does not support the canvas element.


PDF Download: 121

Your browser does not support the canvas element.