Submitted: 28 Sep 2015
Revision: 10 Oct 2015
Accepted: 25 Oct 2015
ePublished: 30 Apr 2016
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Avicenna J Med Biochem. 2016;4(1): 10.
doi: 10.17795/ajmb-33453
  Abstract View: 2082
  PDF Download: 6349
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Research Article

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

Leila Jamalzadeh 1, Hosein Ghafoori 1*, Reyhaneh Sariri 1, Hanieh Rabuti 1, Jila Nasirzade 1, Hajar Hasani 1, Mahmoud Reza Aghamaali 1

1 Department of Biology, University of Guilan, Rasht, IR Iran
*Corresponding author: Hosein Ghafoori, Department of Biology, University of Guilan, Rasht, IR Iran. Tel/Fax: +98-1333333647, h.ghafoori@guilan.ac.ir


Background: Organic solvents are widely used in cell biology experiments. Despite increasing the solubility, they have some moderate toxic effects. Therefore, selecting the appropriate solvent along with the use of suitable concentration insures the accuracy and reliability of experimental results.

Objectives: The current study aimed to examine the cytotoxic effects of some organic solvents on various cell models including MCF-7, RAW-264.7 and human umbilical vein endothelial cells (HUVEC).

Materials and Methods: To evaluate the cytotoxicity effect of common organic solvents on the MCF-7, RAW-264.7 and HUVEC cells, multi-table tournament (MTT) colorimetric assay, the widely used and validated cytotoxicity test was applied. For this purpose, the selected cells were treated with different concentrations (0, 0.1%, 0.5%, 1%, 1.5%, 2%, 3% and5% v/v) of fourmostcommonlyused organic solvents (acetone, ethanol, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) and then subjected to MTT experiment.

Results: According to the obtained results, the cytotoxicity increased significantly with increasing the concentration of all four solvents compared to that of the control group. Studies with MCF-7, RAW-264.7 and HUVEC suggested that acetone, ethanol and DMSO at concentrations of 0.1% and 0.5%, had little or no toxicity, whereas higher concentrations inhibited the growth of all three cells. Compared with other three solvents, DMF displayed rather greater toxicity. Based on the results, proliferation of MCF-7, RAW- 264.7 and HUVEC cells were inhibited by all used organic solvents, dose dependently.

Conclusions: Thus, the background experimental error can be reduced remarkably by maximal concentration of 0.5% ethanol, acetone and DMSO and 0.1% DMF in the final treatment medium.

Keywords: Cytotoxicity, MCF-7, RAW-264.7, HUVEC, Organic Solvents
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