Avicenna J Med Biochem. 2013;1(1):30-35.
Research Article
The arsR
gene: A comparative phylogenetic and bioinformatics analysis to study
reasons for different sensitivity to arsenic compounds
Maryam Daneshpour 1, Nooshin Shabab 2, Massoud Saidijam 2 *
1 Department of Molecular Medicine and Human Genetics, School of Medicine,Hamadan University of Medical Sciences Hamadan, Iran
2 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Abstract
Objectives: The
overall goal of this study was to carry out a set of comparative
analyses of arsR gene in plasmid R773 and bacterial chromosome from
Escherichia coli BL-21(DE3).
Methods: PDB
and NCBI databases and Chimera, Mega4, CLC main workbench software and
3D-jigsaw and EMBL-EBI servers were applied to perform this study. By
using these software and servers, multiple analyses including
determination of residue composition, secondary structure and motifs, 3D
structure, conserved regions, etc. were done.
Results: The
results suggest that such high sensitivity to arsenic compounds in
ars-containing plasmid R773 may be related to ArsR protein
characteristics such as amino acids composition, secondary and tertiary
structure, hy-drophobicity, level of interaction with DNA.
Conclusion: Bioinformatics
studies could be applied to describe the reason of different
sensitivities to Arsenic compounds between arsR gene and ArsR protein in
plasmid R773 and bacterial chromosome.
Keywords: ArsR protein; E coli; Arsenic; Plasmid R773; Protein secondary structure; Protein three dimensional structure