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Submitted: 14 Jul 2013
Revised: 18 Aug 2013
Accepted: 10 Sep 2013
First published online: 28 Sep 2013
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Avicenna J Med Biochem. 2013;1(1):30-35.

Research Article

The arsR gene: A comparative phylogenetic and bioinformatics analysis to study reasons for different sensitivity to arsenic compounds

Maryam Daneshpour 1, Nooshin Shabab 2, Massoud Saidijam 2 *

1 Department of Molecular Medicine and Human Genetics, School of Medicine,Hamadan University of Medical Sciences Hamadan, Iran
2 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Massoud Saidijam Tel: +98 9121324616 Fax: +988118276299 Email: sjam110@yahoo.com

Abstract

Objectives: The overall goal of this study was to carry out a set of comparative analyses of arsR gene in plasmid R773 and bacterial chromosome from Escherichia coli BL-21(DE3).

Methods: PDB and NCBI databases and Chimera, Mega4, CLC main workbench software and 3D-jigsaw and EMBL-EBI servers were applied to perform this study. By using these software and servers, multiple analyses including determination of residue composition, secondary structure and motifs, 3D structure, conserved regions, etc. were done.

Results: The results suggest that such high sensitivity to arsenic compounds in ars-containing plasmid R773 may be related to ArsR protein characteristics such as amino acids composition, secondary and tertiary structure, hy-drophobicity, level of interaction with DNA.

Conclusion: Bioinformatics studies could be applied to describe the reason of different sensitivities to Arsenic compounds between arsR gene and ArsR protein in plasmid R773 and bacterial chromosome.

Keywords: ArsR protein; E coli; Arsenic; Plasmid R773; Protein secondary structure; Protein three dimensional structure