Submitted: 27 Oct 2020
Revision: 13 Nov 2020
Accepted: 16 Nov 2020
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  Abstract View: 31

Research Article

Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats

Forough Kajbaf, Shahrbanoo Oryan, Ramesh Ahmadi* ORCID logo, Akram Eidi


Objective: Growing evidence has shown that the apoptosis of cells plays an important role in the advancement of the diabetic kidney disease. This study attempted to discover the therapeutic potential of Peganum harmala leaf extract in the apoptosis of diabetic kidney disease.
Methods: In the present experimental research, 32 male Wistar rats were studied, and diabetes was induced by streptozotocin (65 mg/kg). The animals were randomly divided into four groups of eight rats each: control, diabetic, control+leaf extract, diabetic+leaf extract. For our purposes, the methanolic extract of P. harmala leaves (150 mg/kg) was given by gavage for 28 days. Flow cytometry and real-time PCR analyses were utilized to determine the percentages of apoptotic cells. Also, histological alterations and blood biochemical parameters were evaluated.
Results: The leaf extract has a high amount of flavonoids (25.84%) and a lower percentage of alkaloids (0.14%) and antioxidant properties. Serum urea (p<0.001) and apoptosis (p<0.05) significantly elevated in diabetic rats relative to the control ones. The mean of fasting blood creatinine, urea, and albumin level was not significantly changed in leaf+ diabetic extract rats as compared to the diabetic ones. Histopathological results also displayed that diabetic complications in the kidney could not be improved following treatment by the leaf extract of P. harmala. In addition, the leaf extract could not significantly reduce the apoptosis and caspase-3 expression compared to diabetics in renal cells.
Conclusion: Based on our findings, the leaf extract of P. harmala is unable to inhibit apoptosis in the diabetic kidney model.
Keywords: Apoptosis, P. harmala, Diabetes, Kidney, Caspase-3,
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Abstract View: 31

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