Submitted: 29 Nov 2021
Revision: 01 Dec 2021
Accepted: 16 Dec 2021
ePublished: 29 Dec 2021
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Avicenna J Med Biochem. 2021;9(2): 65-71.
doi: 10.34172/ajmb.2021.12
  Abstract View: 605
  PDF Download: 415

Research Article

Anti-inflammatory and Antioxidant Properties of PLGA Nanoparticles Produced From Kombucha Extract on A2780 Human Ovarian Cancer Cell Line

Sara Ghandehari 1, Masoud Homayouni Tabrizi 2, Jafar Izadi Nia 3, Mohammad Taghi Goodarzi 4* ORCID logo

1 Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
2 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3 Department of Chemistry, Herbal Medicines Raw Materials Research Center, Shahrood Branch, Islamic Azad University, Shahrood, Iran
4 Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
*Corresponding Author: Corresponding author: Mohammad Taghi Goodarzi, Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran, Tel: +982332390077, Email: , Email: mtgoodarzi@yahoo.com


Background: Anti-cancer agents encapsulated in nanoparticles (NPs) can result in higher efficiency. Kombucha is a fermented tea beverage, and previous reports support its anti-cancer properties.

Objectives: The present study aimed to evaluate the anti-cancer and anti-inflammatory properties of poly (lactic-co-glycolic acid) loaded Kombucha NPs (PLGA-K-NPs) against the A2780 human ovarian cancer cell line.

Methods: The antioxidant activity was analyzed using ferric reducing ability of plasma and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays, along with the measurement of catalase (CAT) gene expression. The gene expression of three interleukins (IL-1β, IL-6, and IL-10) was also determined to demonstrate the anti-inflammatory properties of NPs.

Results: The results revealed the antioxidant effects of PLGA-K-NPs on the studied cell lines by increasing Fe3+ reduction, inhibiting the free radical formation (P<0.001), and increasing the expression of the CAT gene (P<0.001). In addition, NPs could significantly elevate the gene expression of IL-10 (P<0.01) as an anti-inflammatory cytokine at a 40 µg/mL concentration, while reducing the expression of IL-1β and IL-6, and inflammatory cytokines at all tested concentrations (P<0.01).

Conclusion: According to the obtained results, PLGA-K-NPs have anti-inflammatory and anti-oxidant properties, therefore, they can be considered as a compound in the treatment of ovarian cancer. However, it needs to be further investigated in animal studies to clarify more details.

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