Submitted: 17 Jul 2022
Revision: 02 Oct 2022
Accepted: 03 Oct 2022
ePublished: 12 Dec 2022
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Avicenna J Med Biochem. 2022;10(2): 114-119.
doi: 10.34172/ajmb.2022.2375
  Abstract View: 385
  PDF Download: 328

Original Article

Investigating Preventive Effect of Vitamin D and N-acetylcysteine Against Kidney Injury in Rats Versus the Promotive Effect of Paraquat

Hamed Felehgari 1, Nejat Khiripour 2, Fereshteh Mehri 3 ORCID logo, Sara Soleimani Asl 4, Davoud Ahmadimoghaddam 1, Akram Ranjbar 3* ORCID logo

1 Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Clinical Biochemistry, Kashan University Medical Sciences, Kashan, Iran
3 Nutrition Health Research Center, Center of Excellence for Occupational Health, Hamadan University of Medical Sciences, Hamadan, Iran
4 Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author: Corresponding author: Akram Ranjbar, Email: , Email: akranjbar2015@gmail.com


Background: Paraquat (PQ) is one of the most important herbicides used in agriculture.

Objectives: This study was conducted to compare the preventive effect of vitamin D (Vit D) and N-acetylcysteine (NAC) against kidney injury in rats versus the promotive effect of PQ.

Methods: In this study, rats were divided into six groups. The control group (group 1) received normal saline, Vit D group (group 2) received intraperitoneal (IP) injections of+Vit D (2 μg/kg), NAC group (group 3) received NAC (6.25 mg/kg, IP), PQ group (group 4) received PQ (5 mg/kg/d, IP), PQ+Vit D group (group 5) received PQ+Vit D (5 mg/kg/d+2 μg/kg/d, IP) and PQ+NAC group (group 6) received PQ+NAC (5 mg/kg/d+6.25 mg/kg/d, IP). The animals were treated for 7 consecutive days as a sub-chronic exposure. After the collection of urea and serum creatinine, biomarkers of oxidative stress and kidney histopathology were investigated.

Results: PQ increased lipid peroxidation (LPO), urea, and serum creatinine, but it significantly decreased total antioxidant capacity (TAC) and thiol groups. In the groups treated with Vit D and NAC, it was observed that LPO, urea, and creatinine significantly decrease compared with the PQ group, and TAC, thiol groups, and Vit D levels increased in kidney tissue.

Conclusion: The obtained findings revealed that both Vit D and NAC used as preventive compound were able to reduce oxidative stress and tissue damage caused by PQ toxicity in the kidney.

Please cite this article as follows: Felehgari H, Khiripour N, Fereshteh M, Soleimani Asl S, Ahmadimoghaddam D, Ranjbar A. Investigating preventive effect of vitamin d and n-acetylcysteine against kidney injury in rats versus the promotive effect of paraquat. Avicenna J Med Biochem. 2022; 10(2):114-119. doi:10.34172/ajmb.2022.2375
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