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Submitted: 14 Sep 2022
Revision: 11 Oct 2022
Accepted: 12 Oct 2022
ePublished: 11 Dec 2022
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Avicenna J Med Biochem. 2022;10(2): 90-94.
doi: 10.34172/ajmb.2022.2392
  Abstract View: 362
  PDF Download: 225

Original Article

Association of 25 (OH)-D3 Level With the CYP27B1 rs10877012 Variants and Metabolic Parameters in T2DM Patients From Kurdistan, Iraq

Shna Ahmed Mohhamed 1,3 ORCID logo, Zohreh Rahimi 1,2* ORCID logo, Ebrahim Shakiba 1, Fatemeh Khadir 3, Maryam Kohsari 3

1 Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Medical biology Research Center, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
*Corresponding Author: Corresponding author: Zohreh Rahimi, Emails: zrahimi@kums.ac.ir; , Email: rahimizus@yahoo.com

Abstract

Background:There is an increasing prevalence of type 2 diabetes mellitus (T2DM) worldwide. The deficiency of vitamin D is a worldwide health problem that can increase susceptibility to T2DM. Cytochrome P450 family 27 subfamily B member 1 (CYP27B1) encodes the enzyme 1α-hydroxylase in the kidney, which converts 25 (OH)-D3 to 1,25 (OH)2-D3. The CYP27B1 rs10877012 G/T polymorphism is located in the gene promoter and can influence vitamin D3 level.

Objectives: This study aimed to investigate the relationship between CYP27B1 gene polymorphism and the 25 (OH)-D3 serum level and the risk of T2DM. Additionally, the effect of 25 (OH)-D3 level on metabolic parameters was investigated.

Methods: We investigated 310 individuals including 205 T2DM patients and 105 healthy subjects from the city of AL-Sulaymaniyah, Kurdistan of Iraq. The CYP27B1 gene variants were identified by the polymerase chain reaction followed by digestion with HinfI restriction enzyme.

Results: The mean level of 25 (OH)-D3 was 18.4±8.5 ng/mL in T2DM patients and 24.1±9.3 ng/mL in healthy controls (P<0.001). Significantly higher body mass index (BMI), HbA1c level, and fasting blood sugar concentration were detected in individuals with vitamin D insufficiency (P=0.009, P=0.001, and P=0.01, respectively) and vitamin D deficiency (P=0.025, P<0.001, and P<0.001, respectively) compared to those with a sufficient level of vitamin D. The T allele frequency of CYP27B1 was 38.1% in controls and 40.2% in patients (P=0.6). In the presence of the GT genotype, a significantly lower level of 25 (OH)-D3 was obtained compared to the GG genotype (P=0.02) among the patients.

Conclusion: We found that the CYP27B1 rs10877012 polymorphism was not a risk factor for T2DM but it affected the level of 25 (OH)-D3 in diabetic patients and vitamin D deficiency and insufficiency increased the values of BMI, HbA1c, and FBS as the metabolic parameters involved in the development of T2DM.


Please cite this article as follows: Mohhamed SA, Rahimi Z, Shakiba E, Khadir F, Kohsari M. Association of 25 (OH)-D3 level with the CYP27B1 rs10877012 variants and metabolic parameters in T2DM patients from kurdistan, iraq. Avicenna J Med Biochem. 2022; 10(2):90- 94. doi:10.34172/ajmb.2022.2392
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