Submitted: 21 Jun 2023
Revision: 17 Aug 2023
Accepted: 23 Aug 2023
ePublished: 16 Oct 2023
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Avicenna J Med Biochem. 2023;11(2): 129-137.
doi: 10.34172/ajmb.2446
  Abstract View: 243
  PDF Download: 147

Original Article

Structure-Based Virtual Screening of Cinnamic Acid Analogs Against RIPK3: Implications for Anti-Inflammatory Drug Discovery

Elham Khoshbin 1 ORCID logo, Seyed Mohamad Soroosh Rahmani-Abidar 1 ORCID logo, Shadi Moradi 2 ORCID logo, Amir Taherkhani 3* ORCID logo, Hamed Karkehabadi 1* ORCID logo

1 Department of Endodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Medical Immunology, School of Medicine, Hamadan University of Medical Science, Hamadan, Iran
3 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Authors: Amir Taherkhani, Email: amir.007.taherkhani@gmail.com, Email: amir.007.taherkhani@gmail.com; Hamed Karkehabadi, Email: hamed_karkehabadi@yahoo. com, Email: hamed_karkehabadi@yahoo.com


Background: A common oral inflammatory disease known as apical periodontitis (AP) is caused by the intrusion of microorganisms into the dental pulp, resulting in an inflammatory response and bone degradation in periapical tissues. A growing body of evidence indicates that the receptor-interacting serine-threonine kinase 3 (RIPK3) is closely associated with AP.

Objectives: This study sought to address the requirement for effective RIPK3 inhibitors by examining the potential of cinnamic acid natural metabolites capable of inhibiting RIPK3.

Methods: The binding affinity of 20 cinnamic acids to the RIPK3 active site was evaluated by using AutoDock 4.0 software. The most favorable scores were assigned to the highest-ranking cinnamic acids based on their ΔGbinding values to the RIPK3 catalytic domain. A 100-nanosecond (ns) computer simulation was performed using molecular dynamics for the most efficacious inhibitor of RIPK3, and the findings were contrasted with those obtained for free RIPK3. The Discovery Studio Visualizer tool was employed to showcase the interactions between the RIPK3 active site and the highest-ranking metabolites.

Results: The binding affinity of cynarin, rosmarinic acid (RosA), and chlorogenic acid (CGA) to the RIPK3 active site was noteworthy, as the ΔGbinding values were<-10 kcal/mol. Furthermore, cynarin exhibited inhibition constant values at the picomolar range. Upon complexation with cynarin, the RIPK3 conformation attained stability after approximately 25 ns of simulation.

Conclusion: In general, cynarin, RosA, and CGA have the potential to be therapeutically beneficial in treating AP due to their ability to inhibit RIPK3.

Please cite this article as follows: Khoshbin E, Rahmani-Abidar SMS, Moradi S, Taherkhani A, Karkehabadi H. Structure-based virtual screening of cinnamic acid analogs against RIPK3: implications for anti-inflammatory drug discovery. Avicenna J Med Biochem. 2023; 11(2):129-137. doi:10.34172/ajmb.2446
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