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Submitted: 20 Feb 2014
Revision: 14 Apr 2014
Accepted: 21 Apr 2014
ePublished: 25 Sep 2014
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Avicenna J Med Biochem. 2014;2(1): 4-18255.
doi: 10.17795/ajmb-18255
  Abstract View: 2151
  PDF Download: 880
  Full Text View: 1225

Research Article

High Molecular Weight Alkaline Phosphatase Changes Following Animal Copper Treatment

Jamshid Karimi 1*, Ali Asghar Moshtaghie 2, Bahram Haghighi 2

1 Department of Biochemistry and Nutrition, Hamadan University of Medical Sciences, Hamadan, IR Iran
2 Department of Biochemistry, Isfahan University of Medical Sciences, Isfahan, IR Iran
*Corresponding Author: *Corresponding author: Jamshid Karimi, Department of Biochemistry and Nutrition, Hamadan University of Medical Sciences, Hamadan, IR Iran. Tel: +98-8138380462, Fax: +98-8138380208, , Email: jmshdkarimi@yahoo.com

Abstract

Background: Although trace amounts of copper (Cu) are necessary to maintain proper body functions, the excess amount can contribute to the development of hepatic dysfunction.

Objectives: This study aimed to investigate the relationship between copper treatment and changes in the serum concentration of high molecular weight alkaline phosphatase (HMW-ALP).

Materials and Methods: Male Wistar rats were injected intraperitoneally (IP) with copper (Cu) as copper chloride (CuCl2. 4H2O) 4, 2 and 1 mg/kg for 10, 30 and 60 days respectively. Animals were killed at indicated time and blood samples were collected, and sera was separated and used for alkaline phosphatase activity determinations and also for isoenzymes gel filtration chromatography and Sephacryl S-300 was used.

Results: Obtained data showed that with increasing administration of copper, the ALP activity was elevated significantly. In comparison with the control group the elevations were between 20%-56% using gel filtration chromatography. It was found that the elevation of serum ALP was mostly due to HMW-ALP.

Conclusions: The elevation of HMW-ALP activity in Cu treated animal suggests the occurrence of biliary disease. This may be used as a biomarker for the diagnosis of copper toxicity.


Implication for health policy/practice/research/medical education:

 
The elevation of high molecular weight alkaline phosphatase activity during copper (Cu) treatment of animals may suggest the occurrence of biliary disease. This may be used as a biomarker for the diagnosis of copper toxicity.

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