Logo-ajmb
Submitted: 28 Apr 2016
Revision: 18 Jun 2016
Accepted: 20 Jun 2016
ePublished: 02 Aug 2016
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Avicenna J Med Biochem. 2016;4(2): 9-38596.
doi: 10.17795/ajmb-38596
  Abstract View: 2562
  PDF Download: 1862
  Full Text View: 1574

Research Article

Bactericidal Activity of Copper Oxide Nanocomposite/Bioglass for in Vitro Clindamycin Release in Implant Infections Due to Staphylococcus aureus

Zohre Alijanian 1*, Nasrin Talebian 2, Monir Doudi 1

1 Department of Microbiology, Faculty of Biological Science, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, IR Iran
2 Department of Chemistry, Faculty of Biological Science, Shahreza Branch, Islamic Azad University, Shahreza, Isfahan, IR Iran
*Corresponding Author: *Corresponding author: Zohre Alijanian, Department of Microbiology, Faculty of Biological Science, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, IR Iran. Tel: +98-3137420136, , Email: Alijanyan.Z@gmail.com

Abstract

Background: In recent years, bioactive bioceramics such as bioglass and hydroxyapatite (HA) have been introduced as a remarkable  development in the field of medicine due to their bio-adaptability, non-toxicity, and persistence, in vivo. They have many potential applications in the repair of bone defects and hence they have attracted significant interest from scholars.

Objectives: The aim of this study was to synthesize inorganic matrix CuO-based bioglasses and evaluate their antibacterial activity against aerobic bacterial infections in bone implants.

Methods: Nano-composite samples of silica-based bioactive glass, 60SBGwith nano-powderCuO, were synthesized using the sol-gel method and then assessed with regard to their antibacterial properties against Staphylococcus aureus using well diffusion agar. The samples included BG58S (58%SiO2, 36%CaO, 6%P2O5), BG/10CuO (58%SiO2, 26%CaO, 6%P2O5, 10%CuO), and BG/20CuO (48%SiO2, 26%CaO, 6%P2O5, 20%CuO). To evaluate their bioactivity, the prepared samples of BG/20CuO, BG/10CuO, and BG58S were immersed in simulated body fluids (SBF). The surface morphology and structure of the samples before and after immersion in the SBF were characterized using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR), respectively. Then, the BG/20CuO and BG/10CuO samples were loaded in clindamycin, an antibiotic widely used in the treatment of osteomyelitis, and their release profiles were studied in phosphate buffer solution.

Results: It was observed that the growth inhibition zone increased through clindamycin release due to the increasing CuO percentage in the nanocomposite of bioactive glass. The bioactivity of the nanocomposite/bioglass with CuO was superior to that of bioglass alone. In this study, the BG/20CuO sample showed a sustained release of clindamycin, which is sufficient for a drug delivery system.

Conclusions: Increasing the Cu nanoparticles in bioactive glass samples leads to the release of Cu2+, which has a positive effect on the antibacterial mechanism, as well as decreasing the cultured Staphylococcus colonies found on the bioglasses. Therefore, it seems that the nanocomposite/bioglass of CuO is a promising option for aerobic bacterial inhibitor systems in common bone implant infections.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 2563

Your browser does not support the canvas element.


PDF Download: 1862

Your browser does not support the canvas element.


Full Text View: 1574

Your browser does not support the canvas element.