Avicenna J Med Biochem. 2013;1(1):1-6.
Research Article
Effect of flaxseed on serum lipid profile and expression of NPC1L1, ABCG5 and ABCG8 genes in the intestine of diabetic rat
Abbas Mohammadi 1,2, Fateme Mirzaei 3, Mohammad Nabi Moradi 3, Mohammad Jamshidi 3, Reza Yari 4, Tayebeh Ghiasvand 5, Ali Rezaii 6, Ebrahim Abbasi Oshaghi 3 *
1 Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
2 Physiology Research Centre, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
3 Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
4 Department of Biology, Islamic Azad University, Boroūjerd Branch, Boroūjerd, Iran
5 Department of Biochemistry and Nutrition, Hamadan University of Medical Sciences, Hamadan, Iran
6 Departmen of Basic sciences, Tehran Payame Noor University, Tehran, Iran
Ebrahim Abbasi Oshaghi Tel: +98 9126184642 Fax: +98 8118380208 Email: 7abbasi@gmail.com
Abstract
Objectives: The
aim of this study was to examine the effect of flaxseed on gene
expression of intestinal transporters: Niemann-Pick C1 like 1 (NPC1L1),
ATP-cassette binding proteins G5 (ABCG5) and G8 (ABCG8).
Methods: Animals
were randomly divided into 3 groups 8 rats in each group: group1;
normal diet, group2; diabetic rats, and group3; diabetic rats + 4% (w/w)
flaxseed. After one-month rats were sacrificed, blood was collected;
lipid profiles were determined enzymatically, and mRNA levels were
determined by RT-PCR.
Results: Compared
to diabetic rats, flaxseed significantly decreased total cholesterol
(TC), low-density lipoprotein cholesterol (LDL-C), triglycerides, very
low density lipoprotein cholesterol (VLDL-C) and atherogenic index (all
P<0.05). Intestinal NPC1L1 mRNA was significantly decreased
(P<0.01) in flaxseed group treatment compared with diabetic animals.
Intestinal ABCG5 and ABCG8 mRNAs were significantly increased
(P<0.001) in flaxseed group treatment compared with diabetic animals.
Conclusion: In
conclusion, flaxseed significantly reduced lipid profile and
atherogenic index, as compared with the diabetic group. Flaxseed
treatment also led to down-regulation of NPC1L1 mRNA and up-regulation
of ABCG5 and ABCG8 mRNAs in the intestine of rats.
Keywords: ABCG5; ABCG8; Cholesterol; Flax; NPC1L1