vicenna J Med Biochem. 2013;1(1):36-40.
Research Article
In vitro
investigations on the toxicity induced by tamoxifen and
tamoxifen-loaded solid lipid nanoparticles on two breast cancer cell
types
Roghayeh Abbasalipourkabir 1 * , Aref Salehzadeh 2, Rasedee Abdullah 3
1 Department of Biochemistry, Faculty of Medicine, Hamadan University of Medical Science, Hamadan, Iran
2 Department of Entomology, Faculty of Medicine, Hamadan University of Medical Science, Hamadan, Iran
3 Department of Clinical Pathology and Hematology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Malaysia
Abstract
Objectives: Colloidal
drug delivery system, solid lipid nanoparticles (SLNs), helps to
increase the solubility of the drug and its oral bioavailability.
Methods: Tamoxifen
(TAM) as a nonsteroidalantiestrogen drug was formulated in SLN and an
in vitro study was conducted to determine the cytotoxicity effect of
TAM-loaded SLNs on human breast cancer cell lines MCF-7 (estrogen
receptor-positive) and MDA-MB231 (estrogen receptor-negative) cells. The
cytotoxicity was measured by (3-(4,
5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay).
Results: The
results showed that tamoxifen-loaded SLNs has an equally efficient
cytotoxic activity against MCF-7 and MDA-MB231 cells, compared with free
tamoxifen, and the half maximal inhibitory concentration (IC50) of
TAM-loaded SLNs was generally lower than that of free TAM.
Conclusion: This
finding indicates that tamoxifen’s cytotoxicity may result from
improved drug internalization through encapsulation into the SLN matrix
and endocytosis. Therefore, when TAM is incorporated into the SLN
carrier system, its antitumoral activity is still preserved, suggesting
that SLN is a good carrier for the drug insoluble in water.
Keywords: Breast Neoplasms; Nanoparticle; Tamoxifen; Toxicit