Hamadan University of Medical Sciences Avicenna Journal of Medical Biochemistry 2345-4113 9 2 2021 12 29 Inhibition of NF-кB Expression in LPS-Induced RAW264.7 Macrophage Cells by a Thiazolidinone Derivative (TZD-OCH2 CH3 ) 48 53 10.34172/ajmb.2021.09 EN Farahnaz Hasanzadeh Hossein Ghafouri https://orcid.org/0000-0003-2245-0697 Salman Ahmadi Sevda Zarei https://orcid.org/0000-0001-8350-0534 Mahmoud Reza Aghamaali Asadollah Mohammadi Journal Article 10.34172/ajmb.2021.09 2021 07 13 2021 08 22 To date, various derivatives of thiazolidinone in a variety of cell lines have been investigated. The present study aimed to evaluate the toxicity and inhibitory effects of a thiazolidinone derivative called 5-(2,4-bis-4-ethoxy-phenyl azo)-3-hydroxy-benzylidine)-2,4-thiazolidinone (TZD-OCH2CH3) on the expression of NF-кB in LPS-induced RAW264.7 macrophage cell lines. Different concentrations of the MTT assay(0-120 μg/mL) were performed to estimate the biological rate of the cells. The half-maximal inhibitory concentration (IC50) of TZD-OCH2CH3-treated RAW264.7 cells was found to be 115 μg/mL. To determine the inhibitory effect of the synthesized compound on the expression changes of NF-кB, the RAW264.7cells were initially induced with LPS and then treated by 15, 30 and 60 μg/mL of TZD-OCH2CH3. Realtime PCR results confirmed a strong inhibitory effect of TZD-OCH2CH3 on the expression of NF-кB inLPS-induced RAW264.7 cells (IC50 = 48 μg/mL). Overall, these findings suggested that the derivative TZDOCH2CH3 had a significant anti-inflammatory effect.