Hamadan University of Medical Sciences Avicenna Journal of Medical Biochemistry 2345-4113 9 2 2021 12 29 Anti-nociceptive Activity of Quebracho tannin Extract on Pain Induced by Formalin and Writhing Tests in Mice 59 64 10.34172/ajmb.2021.11 EN Fatemeh Zare Shahin Hassanpour https://orcid.org/0000-0002-4417-1819 Ahmad Asghari Alireza Jahandideh Journal Article 10.34172/ajmb.2021.11 2021 07 27 2021 12 15 Background: Based on positive role of the tannins for pain relief, there is no report for possible anti-nociceptive activity of the Quebracho tannin. Objectives: This study aimed to determine the anti-nociceptive activity of the Quebracho tannin extract (QTE) on pain in mice. Materials and Methods: For this purpose, 340 mice were used for formalin and writhing tests each including 4 experiments with 4 sub-groups. In experiment 1, mice were injected with saline, QTE (100 mg/kg), QTE (200 mg/kg), QTE (400 mg/kg), and morphine (5 mg/kg). In the second experiment, injections included saline, QTE (400 mg/kg), naloxone (2 mg/kg), and QTE + naloxone. Experiments 3 and 4 were similar to experiment 2, except that mice injected were with NG-nitro arginine methyl ester (L-NAME, 10 mg/kg) and cyproheptadine (4 mg/kg) instead of naloxone. Then, formalin (1%) was injected, and time spent for licking the injected paw was recorded until 30 minutes following injection in the first and second phases. Finally, injections in 4 experiment groups were the same, and animals were intraperitoneally injected with acetic acid, and contractions were recorded in the writhing test category. Results: According to the results, QTE (100, 200, and 400 mg/kg) decreased pain in the injected paw (P=0.001) and inhibited the pain response by 59.37% (P=0.001). Moreover, the injection of naloxone + QTE significantly decreased pain in the injected paw (P=0.021). Eventually, the injection of the L-NAME + QTE significantly reduced the anti-nociception effect of the QTE on the formalin test (P=0.031) and writhing contractions (55.75%, P=0.033). Conclusion: These findings suggested anti-nociceptive properties of the QTE mediated by opioidergic and nitrergic systems.