Hamadan University of Medical Sciences Avicenna Journal of Medical Biochemistry 2345-4113 11 1 2023 06 29 Qualitative and Quantitative Analysis of Nepeta leucostegia Essential Oil Components by GC–MS/FID Along With Antioxidant Activity 55 59 10.34172/ajmb.2023.2433 EN Baram Hamah Ameen https://orcid.org/0000-0003-4456-9795 Hiva Ghaderi Dara Dastan https://orcid.org/0000-0002-7208-208X Journal Article 10.34172/ajmb.2023.2433 2023 04 05 2023 05 02 Background: Nepeta is a large genus belonging to the Lamiaceae family. So far, no study has been reported on Nepeta leucostegia compounds and biological effects. Objectives: In the present study, we aimed to evaluate the qualitative and quantitative analysis of Nepeta leucostegia essential oil components along with antioxidant activity. Methods: Essential oil compounds of N. leucostegia were qualitatively and quantitatively identified and analyzed using gas chromatography–mass spectrometry (GC-MS) and GC with flame ionization detection (GC-FID) analysis. Besides, the evaluation of the antioxidant property was performed using DPPH assay. Results: After analysis, 96.63% of essential oil compounds were identified. Among the 36 identified compounds, 1,8-cineole (39.1%), epi-α-cadinol (6.9%), α-Terpineol (6.0%), α-fenchene (5.8%), and Camphene (5.5%) were the main compounds, respectively. The essential oil showed a significant antioxidant effect (IC50=75 μg/mL). Conclusion: The results of this study complete the information on the Nepeta genus and can be used in the chemotaxonomic study of the Nepeta genus. Due to its antioxidant effect, N. leucostegia has the potential for use in pharmaceutical and food industries.