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Submitted: 07 Apr 2020
Accepted: 15 Dec 2020
ePublished: 30 Dec 2020
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Avicenna J Med Biochem. 2020;8(2): 89-93.
doi: 10.34172/ajmb.2020.13
  Abstract View: 135
  PDF Download: 73

Research Article

Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran

Roya Moloudinia 1, Gelavij Mahmoodi 2, Mohammad Abdi 3,4, Sabrieh Amini 1*, Shirin Ferdowsi 5

1 Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2 Department of Biology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
3 Cellular and Molecular Research Center, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
4 Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
5 Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
*Corresponding author: Sabrieh Amini, PhD, Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran Email: amini.biology@gmail. com

Abstract

Background: Several studies have indicated that polymorphism in vitamin D pathway genes is associated with breast cancer (BC) risk. Vitamin D-binding protein (VDBP) is a vital element in the metabolism of the vitamin D. VDBP carries the serum 25(OH) D3 to cells to promote vitamin D biological functions, such as cell proliferation and apoptosis. Missense SNP (rs.7041) is a common polymorphism in VDBP gene, which shows ethnic-specific allele frequencies.

Objectives: This study presents the correlation of the rs7041 (Asp432Glu) gene polymorphism and plasma concentrations of VDBP in Kurdish patients with BC in Sanandaj, Iran.

Methods: This cross-sectional study included 44 premenopausal BC patients and 44 healthy subjects. Plasma VDBP concentration was measured by enzyme-linked immunosorbent assay (ELISA). The VDBP (rs7041) was genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP).

Results: VDBP level was associated with a non-significant risk of BC (P=0.397). Frequencies of individuals with VDBP (rs7041) TT, TG, and GG genotypes were 13.6%, 52.2%, and 34.09% in case group and 11.3%, 79.5%, and 9.9% in control group, respectively. Genotype GG associated with increased susceptibility to developing BC (odds ratio [OR]=5.172, CI: 1.555-17.2, P=0.007). There was a significant reverse correlation between GT genotype and BC (OR=0.282, 95% CI: 0.110-0.722, P=0.008)

Conclusion: The changes in the vitamin D pathway may increase susceptibility to develop BC in the Iranian Kurdish population.


Keywords: Polymorphism, Vitamin D binding protein, breast cancer,
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