Farahnaz Hasanzadeh
1, Hossein Ghafouri
2,3* 
, Salman Ahmadi
2, Sevda Zarei
2 , Mahmoud Reza Aghamaali
2, Asadollah Mohammadi
41 Department of Biology, Faculty of Basic Sciences, University of Guilan, University Campus 2, Rasht, Iran
2 Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran
3 Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran
4 Department of Chemistry, Faculty of Sciences, University of Guilan, Rasht, Iran
*Corresponding Author: *Corresponding author: Hossein Ghafouri, Associate Professor in Biochemistry, Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran. Tel: +981333333647; Email: , Email:
h.ghafoori@guilan.ac.ir
Abstract
To date, various derivatives of thiazolidinone in a variety of cell lines have been investigated. The present study aimed to evaluate the toxicity and inhibitory effects of a thiazolidinone derivative called 5-(2,4-bis-4-ethoxy-phenyl azo)-3-hydroxy-benzylidine)-2,4-thiazolidinone (TZD-OCH2CH3) on the expression of NF-кB in LPS-induced RAW264.7 macrophage cell lines. Different concentrations of the MTT assay(0-120 μg/mL) were performed to estimate the biological rate of the cells. The half-maximal inhibitory concentration (IC50) of TZD-OCH2CH3-treated RAW264.7 cells was found to be 115 μg/mL. To determine the inhibitory effect of the synthesized compound on the expression changes of NF-кB, the RAW264.7cells were initially induced with LPS and then treated by 15, 30 and 60 μg/mL of TZD-OCH2CH3. Realtime PCR results confirmed a strong inhibitory effect of TZD-OCH2CH3 on the expression of NF-кB inLPS-induced RAW264.7 cells (IC50 = 48 μg/mL). Overall, these findings suggested that the derivative TZDOCH2CH3 had a significant anti-inflammatory effect.