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Submitted: 13 Jul 2021
Revision: 22 Aug 2021
Accepted: 22 Aug 2021
ePublished: 29 Dec 2021
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Avicenna J Med Biochem. 2021;9: 48-53.
doi: 10.34172/ajmb.2021.09
  Abstract View: 205
  PDF Download: 119

Research Article

Inhibition of NF-кB Expression in LPS-Induced RAW264.7 Macrophage Cells by a Thiazolidinone Derivative (TZDOCH2CH3)

Farahnaz Hasanzadeh 1, Hossein Ghafouri 2,3* ORCID logo, Salman Ahmadi 2, Sevda Zarei 2 ORCID logo, Mahmoud Reza Aghamaali 2, Asadollah Mohammadi 4

1 Department of Biology, Faculty of Basic Sciences, University of Guilan, University Campus 2, Rasht, Iran
2 Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran
3 Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran
4 Department of Chemistry, Faculty of Sciences, University of Guilan, Rasht, Iran
*Corresponding Author: *Corresponding author: Hossein Ghafouri, Associate Professor in Biochemistry, Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran. Tel: +981333333647; Email: , Email: h.ghafoori@guilan.ac.ir

Abstract

To date, various derivatives of thiazolidinone in a variety of cell lines have been investigated. The present study aimed to evaluate the toxicity and inhibitory effects of a thiazolidinone derivative called 5-(2,4-bis-4-ethoxy-phenyl azo)-3-hydroxy-benzylidine)-2,4-thiazolidinone (TZD-OCH2CH3) on the expression of NF-кB in LPS-induced RAW264.7 macrophage cell lines. Different concentrations of the MTT assay(0-120 μg/mL) were performed to estimate the biological rate of the cells. The half-maximal inhibitory concentration (IC50) of TZD-OCH2CH3-treated RAW264.7 cells was found to be 115 μg/mL. To determine the inhibitory effect of the synthesized compound on the expression changes of NF-кB, the RAW264.7cells were initially induced with LPS and then treated by 15, 30 and 60 μg/mL of TZD-OCH2CH3. Realtime PCR results confirmed a strong inhibitory effect of TZD-OCH2CH3 on the expression of NF-кB inLPS-induced RAW264.7 cells (IC50 = 48 μg/mL). Overall, these findings suggested that the derivative TZDOCH2CH3 had a significant anti-inflammatory effect.
Keywords: Thiazolidinone, NF-кB, RAW264.7 cells, LPS- induced, Inflammatory pathways,
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