Logo-ajmb
Submitted: 08 Jan 2022
Revision: 28 Feb 2022
Accepted: 07 Mar 2022
ePublished: 25 Jun 2022
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Avicenna J Med Biochem. 2022;10: 37-45.
doi: 10.34172/ajmb.2022.05
  Abstract View: 220
  PDF Download: 111

Original Article

Proteolytic Sensitivity, In Vitro Glycation Efficiency of Diabetic and Non-diabetic Human Serum Albumin

Hadiya Husain, Riaz Ahmad* ORCID logo

1 Section of Genetics, Department of Zoology, Faculty of Life Science, Aligarh Muslim University, Aligarh-202001, India
*Corresponding Author: Corresponding author: Dr. Riaz Ahmad, Section of Genetics, Department of Zoology, Faculty of Life Science, Aligarh Muslim University, Aligarh-202001, India Tel: +91-571-2700920-3445, Tel: +91-571-2700920-3445, Email: , Email: ahmadriaz2013@gmail.com

Abstract

Background: Glycation of human serum albumin (HSA) leads to disturbances in its stability, activity, and other properties which, in turn, affect the functional properties of HSA. Modification of albumin by glycation shows considerable potential as a significant biochemical biomarker for diagnosing diabetes. The characteristics of the glycation process in proteins have not been fully examined yet and, therefore, there is insufficient knowledge about them in the field.

Objectives: This study aimed to clarify the differences between diabetic and non-diabetic HSA as well as their structure-function relationship.

Methods: The physiological and laboratory characteristics of glycated albumin as well as HSA were explored. A total of 30 subjects were enrolled in this study in which 15 normal healthy individuals were assigned into the control group, and 15 type-2 diabetic patients were included in the diabetic group. Patients with type-1 diabetes, pregnant women, and individuals with other diseases were excluded from the study. Protein estimation, polyacrylamide gel electrophoresis, ammonium sulphate fractionation, dialysis, glycation of HSA followed by gel electrophoresis of glycated samples, digestion of BSA, as well as HSA by α-chymotrypsin and their documentation and stoichiometry were all performed.

Results: Various characteristic differences were observed between diabetic and non-diabetic HSA including proteolytic susceptibility and in vitro glycation efficiency. Hypoalbuminemia was, particularly, observed in diabetic patients, which was suggestive of a relationship between hyperglycemia and hypoalbuminemia.

Conclusion: Peculiar contrariety between diabetic and non-diabetic HSA, specific differences in their glycation efficiencies, as well as proteolytic susceptibility and their innuendos were precisely traced out. It was concluded that albumin may have been regarded as a significant clinical biomarker for diagnosing diabetes.

Keywords: Diabetes, Human serum albumin, Glycation, Hypoalbuminemia, Proteolytic susceptibility,

Please cite this article as follows: Husain H, Ahmad R. Proteolytic sensitivity, in vitro glycation efficiency of diabetic and non-diabetic human serum albumin. Avicenna J Med Biochem. 2022; 10(1):37-45. doi:10.34172/ajmb.2022.05
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 198

Your browser does not support the canvas element.


PDF Download: 111

Your browser does not support the canvas element.