Submitted: 16 Dec 2015
Accepted: 19 Dec 2015
First published online: 07 Jun 2016
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

Avicenna J Med Biochem. 2016;4(2): e35557.
doi: 10.17795/ajmb-35557
  Abstract View: 381
  PDF Download: 399
  Full Text View: 367

Research Article

The Effect of Silver Nanoparticles on the Biochemical Parameters of Liver Function in Serum, and the Expression of Caspase-3 in the Liver Tissues of Male Rats

Mahsa Pourhamzeh 1, Zahra Gholami Mahmoudian 1, Massoud Saidijam 2, Mohamad Javad Asari 3, Zohreh Alizadeh 4 *

1 Students Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
2 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
3 Department of Environmental and Occupational Health, School of Public Health, Hamadan University of Medical Sciences, Hamadan, IR Iran
4 Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
*Corresponding author: Zohreh Alizadeh, Department of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran. Tel: +98-9181110700, Email:


Background: Silver nanoparticles have antibacterial properties and their use is growing in different industries. Since the toxicity of nanosilver is not well known, it is essential to examine its safety.

Objectives: This experiment was undertaken to study the effects of nanosilver on rat liver function with an evaluation of blood biochemistry parameters and caspase-3 expression in the liver.

Materials and Methods: In this experimental study, 40 adult male Sprague-Dawley rats were divided into five groups. In the four experimental groups, nanosilver particles were given orally for 28 consecutive days at doses of 30, 125, 300, or 700 mg/kg. Rats in the control group received equal volumes of deionized water. To evaluate the expression of caspase-3 in liver tissue, the real-time PCR method was used. Albumin, total protein, total bilirubin, alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase were measured with an RA-1000 autoanalyzer.

Results: The results indicated that caspase-3 was upregulated in the treated groups compared to the control group (P < 0.05). No considerable changes in serum biochemical parameters were observed (P > 0.05).

Conclusions: Based on the present study, it can be concluded that oral administration of silver nanoparticles for 28 days had no effect on rat liver function, but probably led to early apoptotic stages.

First name
Last name
Email address
Security code