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Submitted: 20 Mar 2020
Accepted: 19 Jun 2020
ePublished: 29 Jun 2020
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Avicenna J Med Biochem. 2020;8(1): 15-20.
doi: 10.34172/ajmb.2020.02
  Abstract View: 745
  PDF Download: 380

Research Article

Preliminary Nutrient Determination and Regeneration of Pancreatic Islet Cells by Extracts of Spondias mombin Leaves in Streptozotocin-Induced Diabetic Rats

Nkeiruka Eluehike 1* ORCID logo, Iyere Osolase Onoagbe 2 ORCID logo

1 Department of Medical Biochemistry, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria.
2 Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria.
*Corresponding Author: *Corresponding author: Nkeiruka Eluehike, Department of Medical Biochemistry, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria. Tel: +2348061344256, Email:, Email: nkeiruka.ezeugwu@ uniben.edu

Abstract

Background: There is a growing trend towards the use of medicinal plant that can prevent degeneration of pancreatic cells and regenerate islet cells in diabetes treatment

Objectives: The possible effects of the extracts of Spondias mombin leaves on the regeneration of pancreatic beta cells in streptozotocin (STZ)-induced diabetic rats were determined in this study.

Methods: Twenty-five male Wistar rats were randomly divided into 5 groups (A-E) including normal and diabetic rats. Aqueous and ethanol extracts of S. mombin were administered orally to diabetic rats in groups D and E for 12 weeks. Blood samples were collected for serum insulin and C-peptide assays. The pancreases were also collected for histopathological examination. Additionally, mineral and vitamin contents of the leaves were determined using standard procedures.

Results: The highest vitamin content was vitamin C in the leaves, while the highest mineral content was calcium. Treatment with extracts of Spondias mombin attenuated the STZ-induced hypoinsulinemia, which was evident from the increased serum insulin and c-peptide levels in the extract treated groups. Histopathological examination of the pancreas revealed an increase in the number and size of the pancreatic beta cells in the extracts treated groups in comparison to the shrunken and diminished islet cells observed in the untreated animals.

Conclusion: Extracts of S. mombin leaves are capable of regenerating pancreatic β-cells which were destroyed by streptozotocin induction.

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Abstract View: 746

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